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The exon junction complex differentially marks spliced junctions

Saulière Jérôme, Haque Nazmul, Harms Scott, Barbosa Isabelle, Blanchette Marco and Le Hir Hervé

The exon junction complex (EJC), which is deposited onto mRNAs as a consequence of splicing, is involved in multiple post-transcriptional events in metazoa. Here, using Drosophila melanogaster cells, we show that only some introns trigger EJC-dependent nonsense-mediated mRNA decay and that EJC association with particular spliced junctions depends on RNA cis-acting sequences. This study provides the first evidence to our knowledge that EJC deposition is not constitutive but instead is a regulated process.

Nat Struct Mol Biol. 2010 Oct ;17(10):1269-71.