Post-doctoral position to study cerebellar circuit dynamics
We are recruiting a motivated postdoctoral fellow to study cerebellar circuit dynamics using optical voltage recording at the cellular level in behaving mice. Our team has developed ULoVE1 and 3D-CASH2 technologies and is the world leader for two-photon recording of Genetically Encoded Voltage Indicators (GEVIs)1,3, which is performed routinely in the lab on cell populations in vivo. This project is centered on the study of inhibitory microcircuits in the context of pattern separation.
Requirements
Candidates will have performed in vivo multiphoton optical recordings or targeted patch-clamp recordings, must be skilled in programing (data analysis) and familiar with viral transduction and associated genetic strategies.
This position is funded by an ANR grant for 2 years and covers all experimental costs.
References
1. Villette, V. et al. Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice. Cell 179, 1590-1608.e23 (2019).
2. Akemann, W. et al. Fast optical recording of neuronal activity by three-dimensional custom-access serial holography. Nat Methods 19, 100–110 (2022).
3. Liu, Z. et al. Sustained deep-tissue voltage recording using a fast indicator evolved for two-photon microscopy.
Cell S0092867422009163 (2022) doi:10.1016/j.cell.2022.07.013.
Our lab, located in the Institut de Biologie de l’Ecole Normale Supérieure in Paris Latin Quarter, provides a rich and vibrant experimental and training environment, in addition to all the required facilities (animal breeding, virus production, imaging, FabLab), to embark on this project. The team is highly multidisciplinary, combining expertise ranging from state-of-the-art molecular biology to physiology, optics and instrumental development. The selected candidate will have full access to unique ultrafast random-access multiphoton microscopy, developed in the lab in the past decade.
Potential applicants should contact Vincent Villette at vincent.villette chez bio.ens.psl.eu with their CV, cover letter, and contact information of one or more academic references.
